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Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 87-96 doi: 10.15302/J-FASE-2016087
关键词: safety of transgenic bicistron gene transfer body transgenic mice muscle development
Functional characterization of caffeic acid
Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU
《农业科学与工程前沿(英文)》 2018年 第5卷 第1期 页码 98-107 doi: 10.15302/J-FASE-2017198
关键词: biofuel crop caffeic acid O-methyltransferase forage lignin Panicum virgatum switchgrass transgenic plant
官春云
《中国工程科学》 2002年 第4卷 第8期 页码 34-39
简要介绍了国外油菜转基因育种情况,包括转除草剂抗性基因,病原菌抗性基因,耐重金属基因, 影响种子贮藏产物基因,影响繁殖特性基因,医药和工业产品基因,以及启动子调节基因等。较详细介绍了湖 南农业大学在Bt毒蛋白基因转化甘蓝型油菜育成抗虫新品系、基因工程雄性不育系、恢复系选育和杂种优势利 用,以及反义FAD2基因转化甘蓝型油菜双低品种提高油酸含量的研究结果;浙江省农科院利用反义PEP基因 转化甘蓝型油菜提高种子含油量的研究结果。
胡炜,朱作言
《中国工程科学》 2016年 第18卷 第3期 页码 105-109 doi: 10.15302/J-SSCAE-2016.03.018
2015年11月19日,美国食品药品监督管理局(FDA)批准了转全鱼生长激素基因(gh)大西洋鲑为第一种可供食用的转基因动物产品,从而取得世界转基因动物育种研究与产业化的历史性突破。经过了长达20年的严格审核,转gh大西洋鲑才获准产业化,不仅充分说明了对包括转基因鱼在内的创新产业的管理决策尤其需要尊重科学规律和客观事实;而且表明基础研究只是产业创新的因素之一,转基因知识的科学普及与企业的积极参与是推动高新技术发展和创新产业形成不可或缺的重要条件;高屋建瓴地制定我国基因改良农业品种市场准入的条例和操作细则已经刻不容缓。
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 66-72 doi: 10.15302/J-FASE-2018229
Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes huge economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to diminish PEDV and TGEV infection rates. RNA interference has been applied successfully to inhibit the virus replication. It provides a potential strategy for breeding resistant pigs. In this study, four promoters and four short hairpin RNA (shRNA) vectors with LoxP sites at each end of the selectable marker genes were constructed to target PEDV and TGEV. These vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNT porcine blastocysts were obtained. These results have paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT.
关键词: piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 179-184 doi: 10.15302/J-FASE-2014020
关键词: recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic mice milk
IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE -2020343
The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.
关键词: Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis
ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE-2020343
关键词: Botrytis cinerea food preservation plant diseases plant pathogenic fungi Tupistra chinensis
Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO
《农业科学与工程前沿(英文)》 2014年 第1卷 第2期 页码 158-173 doi: 10.15302/J-FASE-2014015
关键词: bovine Ig μ heavy-chain transgenic mice B cell development allelic exclusion immune response Ig repertoire
An introduction to the medicinal plant genome project
Shilin Chen, Li Xiang, Xu Guo, Qiushi Li
《医学前沿(英文)》 2011年 第5卷 第2期 页码 178-184 doi: 10.1007/s11684-011-0131-0
HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES
《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 页码 98-109 doi: 10.15302/J-FASE-2021389
About 80% of plant viruses are transmitted by specific insect vectors, especially hemipterans with piercing-sucking mouthparts. Many virus-transmitting insects are also important crop pests that cause considerable losses in crop production. This review summarizes the latest research findings on the interactions between plant viruses and insect vectors and analyzes the key factors affecting insect transmission of plant viruses from the perspectives of insect immunity, insect feeding, and insect symbiotic microorganisms. Additionally, by referring to the latest applications for blocking the transmission of animal viruses, potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology, and CRISPR/Cas9+ gene-driven technology are discussed.
关键词: control strategies / feeding / immunity / insect vector / microorganism / plant virus
Intelligent diagnosis methods for plant machinery
Huaqing WANG, Peng CHEN, Shuming WANG,
《机械工程前沿(英文)》 2010年 第5卷 第1期 页码 118-124 doi: 10.1007/s11465-009-0084-z
关键词: intelligent diagnosis neural network fuzzy algorithm adaptive filtering plant machinery
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
《农业科学与工程前沿(英文)》 2016年 第3卷 第2期 页码 102-112 doi: 10.15302/J-FASE-2016100
关键词: plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network
Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project
Oluwole Alfred OLATUNJI
《工程管理前沿(英文)》 2018年 第5卷 第3期 页码 334-346 doi: 10.15302/J-FEM-2018011
This paper uses project organizational theories to draw lessons from a historic megaproject, the Ajaokuta Steel Plant (ASP). Archival reports on the ASP were explored to identify the unique attributes of the project; the political wrangling that underplayed its evolution, its economic significance and organizational impacts. Findings suggest the goals of the ASP project were, and still are, unambiguous. Failure occurred as socio-political forces aggravated the project’s complex milestones. Stakeholders were impatient with pre-project investigations. During planning, owners ignored opinions that were contrary to their expectations. While delays lingered, pressures from the global economy weakened the project’s motivation to succeed. These combined to turn the project’s outcomes into a chaotic situation that triggered dire implications. Despite about 1400% overrun in cost, the success achieved on the plant was 28% at commissioning. Contractors remained on site until eight years after commissioning. Six key elements of the 482 items in the ASP project contract were not delivered nearly 40 years on. A simplistic look at these suggests poor planning is the main problem. However, planning issues is not entirely strange in greenfield projects. The paper draws strength from project organization theories to explain what was poor about the planning. Socrates’ generic management theory was used to explain the role of leadership in the failure of the ASP project. McGregor’s Theory X and Theory Y explain the significance of stakeholders’ integration in megaprojects. Systems and chaos theories were used to explain the sensitivity of the ASP project to uncertainties. Narratives on these combine well to inspire stakeholders of megaprojects on where and how to seek courage in making effective plans that can help achieve success in complex projects. While normative literature only recognizes project success in a definitive perspective, this study provides insights from failure as an instrument to trigger sublime reflections.
关键词: industrial projects megaprojects Nigeria project organizational theories steel plant
Rhizosphere immunity: targeting the underground for sustainable plant health management
Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN
《农业科学与工程前沿(英文)》 2020年 第7卷 第3期 页码 317-328 doi: 10.15302/J-FASE-2020346
Managing plant health is a great challenge for modern food production and is further complicated by the lack of common ground between the many disciplines involved in disease control. Here we present the concept of rhizosphere immunity, in which plant health is considered as an ecosystem level property emerging from networks of interactions between plants, microbiota and the surrounding soil matrix. These interactions can potentially extend the innate plant immune system to a point where the rhizosphere immunity can fulfil all four core functions of a full immune system: pathogen prevention, recognition, response and homeostasis. We suggest that considering plant health from a meta-organism perspective will help in developing multidisciplinary pathogen management strategies that focus on steering the whole plant-microbe-soil networks instead of individual components. This might be achieved by bringing together the latest discoveries in phytopathology, microbiome research, soil science and agronomy to pave the way toward more sustainable and productive agriculture.
关键词: rhizosphere soil microbiome plant immunity microbial ecology plant health soilborne pathogens
标题 作者 时间 类型 操作
regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice
Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
期刊论文
Functional characterization of caffeic acid
Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU
期刊论文
Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
期刊论文
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
期刊论文
ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG
期刊论文
conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic
Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO
期刊论文
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
期刊论文
Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project
Oluwole Alfred OLATUNJI
期刊论文